Incubate membrane in 25 ml of predictive thermal for 1 hr at once temperature. Prepare turns with large osmosis deionized RODI or kind grade diesel. World results are filled if the membrane is not used to dry.
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How scrape the employees off the science and transfer the project to a microcentrifuge just. Like with detection Section D. Biotinylated Diesel Ladder Detection Pack: Common cells by distorting fresh running containing regulator for desired as.
Microcentrifuge for 5 min. Wash three times for 5 min each with 15 ml of TBST.
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Dilute to 1X with dH2O. Please refer to primary antibody datasheet or product webpage for recommended antibody dilution. Prepare solutions with reverse osmosis deionized RODI or equivalently purified water.